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. 2021 Feb 26;11:4763. doi: 10.1038/s41598-021-83875-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Characterization of Sia9N₃ induced fluorescence staining with flow cytometry and confocal fluorescence microscopy. Labeling of SA-presenting microbes is Sia9N₃ (a) and DBCO-BTN (b) dose-dependent. (c) Addition of excess natural sialic acid (SA) partially competed for fluorescence labeling ([Sia9N3]: 200 μM, [SA]: 1000 μM). (d) Confocal fluorescence microscopic imaging showed that Sia9N₃ induced fluorescence labeling on the bacterial cell surface.