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. 2021 Feb 26;11:4763. doi: 10.1038/s41598-021-83875-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Bacterially incorporated surface Sia9N₃ can be removed by microbial sialidases over time. (a) Treatment of labeled microbes before DBCO-BTN conjugation with a recombinant sialidase significantly reduces fluorescence labeling. The incorporated Sia9N₃ is removed by microbial sialidases over time as fluorescence labeling was gradually removed during microbiota cultivation (b) and microbial sialidase activity was constantly detected in the culture media, 500 pM recombinant BT0455 was used as a positive control (c). (d) 16S rRNA gene sequencing revealed the change of taxonomic composition during H2 microbiome culture. “Bkg” stands for background, “rSialidase” stands for recombinant sialidase.