Table 1.
Plasmids used in this study.
| Plasmid | Description | Reference or source |
|---|---|---|
| pFL38 | CEN-ARS (URA3) | 51 |
| pFL36 | CEN-ARS (LEU2) | 51 |
| pAS103 | YEp-HA-NPR1 (URA3) | 29 |
| pHl-U | YEp-ADH1p-pHluorin (URA3) | 52,53 |
| pPS15-P1 | YCp-(Sc)PMA1 (LEU2) | 54 |
| pPMA4882ochre | YEp-PMA1p-(Np)PMA4882ochre (LEU2) | 11 |
| pPMA2E14D | YEp-PMA1p-6xHis-(Np)PMA2E14D (LEU2) | 11 |
| pES150 | YEp-PMA1p-6xHis-(Np)PMA2E14D-Δ(887-956) (LEU2) | This study |
| pCF053 | YEp-PMA1p-6xHis-(Np)PMA2E14D-Δ(880-956) (LEU2) | This study |
| pCF052 | YEp-PMA1p-6xHis-(Np)PMA2E14D-Δ(865-956) (LEU2) | This study |
| pES162 | YEp-PMA1p-6xHis-(Np)PMA2E14D-T955A (LEU2) | This study |
| pES164 | YEp-PMA1p-6xHis-(Np)PMA2E14D-S938A (LEU2) | This study |
| pES166 | YEp-PMA1p-6xHis-(Np)PMA2E14D-S938D (LEU2) | This study |
| pES168 | YEp-PMA1p-6xHis-(Np)PMA2E14D-T931A (LEU2) | This study |
| pES170 | YEp-PMA1p-6xHis-(Np)PMA2E14D-T931D (LEU2) | This study |
| pES173 | YCp-6xHis-5xGA-(Sc)PMA1 (LEU2) | This study |