Fig. 6. Activation properties of Sl/AoHv1 chimeric channel with swaps in S1–S2 and at S3–S4 loops.
a G–V curve of ChL1-2b + L3-4 chimera compared to parent channels SlHv1 and AoHv1 (pHi = pHo = 6.0). Conductance of chimera starts increasing within the voltage range of AoHv1 activation (blue-shaded area) and reaches maximum within the voltage range of SlHv1 activation (orange-shaded area). G–V parameters derived from Boltzmann fit of the data are: V1/2 = 12.7 ± 1.7 mV, slope = 13.9 ± 1.3 mV (n = 5). b Time constant of activation (τon) and half-maximal deactivation time (t1/2) of ChL1-2b + 3–4 compared to parent channels SlHv1 and AoHv1. τon was measured at 60 mV and t1/2 was measured at −80 mV. Bars are mean values from 4 to 5 independent measurements ± SEM. A one-way ANOVA multiple comparison test with Tukey’s post hoc correction was used for statistical analysis: *p < 0.05, ***p < 0.001.