Figure 2.

Enzyme-linked immunosorbent assay (ELISA) showing high-titer antibodies induced in recombinant BB0405 immunized mice. BB0405 specific total IgG responses were measured in mice sera of three vaccination groups with 8 mice each. IgG responses are presented as optical density (OD) 450–655 nm for multiple sera dilutions. The first group was immunized at three time points (0, 14 and 28 days) with recBB0405. The second groups received DNA vaccinations at the same time points with DNA BB0405 and the third group with DNA Empty (negative control). Plates were coated with recombinant BB0405 and incubated with mouse sera, which was collected from mice at time point 42 days, just before tick challenge and after three vaccinations. Sera was diluted in steps of 300 until 1: 218700.