Table 1.

Overview of number of Borrelia positive mice per group in qPCR tissues and culture.

Vaccin	qPCR positivity					Culture positivity		Cumulative positivity
	Skin	Ear	Bladder	Heart	Ankle	Skin	Bladder
Rec BB0405	4/8	6/8	2/8	4/8	6/8	4/8	4/8	8/8
DNA BB0405	3/8	4/8	1/8	2/8	4/8	2/8	2/8	5/8
Control	5/8	4/8	4/8	3/8	3/8	3/8	2/8	8/8

Each experimental group consisted of 8 mice. qPCR was performed on 5 different tissues: skin (tick bite site on the back), ear, bladder, heart and ankle. Both skin and half of the bladder were cultured in MKP medium and checked for growth of Borrelia for 8 weeks. Statistical significance of cumulative data of the experimental groups compared to the DNA Empty was calculated with the Fisher’s exact test. Comparison between all separate groups was not statistically significant.