Fig. 1. Age-associated muscle atrophy in Mib1^ΔMF mice.

a, b Immunoblotting (IB) of Mindbomb-1 (Mib1) from wild-type (WT) gastrocnemius (GA) muscles at indicated ages (a) and quantification of western blot analysis (b). The intensity of Mib1 expression at indicated ages (M; month) was quantified by densitometry. c–f Body weights (c) and relative tibialis anterior (TA) (d), GA (e), and quadricep (Q) (f) muscle masses to body weights of Mib1^f/f (Mib1^WT) and MCK-Cre;Mib1^f/f (Mib1^ΔMF) at indicated ages. g Gross morphology of hindlimb muscles (TA, GA, and Q muscles) from 16-month-old Mib1^WT and Mib1^ΔMF mice. Scale bars, 0.5 cm. h–j Inguinal (Ing) fat (h), epididymal (Epi) fat (i), and visceral (Vis) fat (j) masses to body weights of Mib1^WT and Mib1^ΔMF at indicated ages. k Gross morphology of fat (Ing, Epi, and Vis fats) from 16-month-old Mib1^WT and Mib1^ΔMF mice. Scale bars, 1 cm. Data are presented as means ± s.e.m. Data are shown as representatives of at least three independent experiments. n = 4 (b), n = 11, 18, and 17 for 3-, 9-, and 16-month-old Mib1^WT mice and 16, 18, and 16 for 3-, 9-, and 16-month-old Mib1 ^ΔMF mice, respectively (c); n = 8, 3, and 7 for 3-, 9-, and 16-month-old Mib1^WT mice and 7 for 3-, 9-, and 16-month-old Mib1 ^ΔMF mice, respectively (d–f, h–j); n = 7, 3, and 6 for 3-, 9-, and 16-month-old Mib1^WT mice and n = 7, 4, and 6 for 3-, 9-, and 16-month-old Mib1 ^ΔMF mice, respectively (d–f, h–j). One-way ANOVA for (b). Two-way ANOVA for (c–f, h–j). *P < 0.05; ***p < 0.001.