Fig. 4. Hes1 but not MyoD is required for the oscillatory expression of Dll1.

a Bioluminescence signals from Dll1-luciferase observed in a single MyoD^−/− mutant muscle stem cell associated with a myofiber and a quantification of this bioluminescence signal (left); the myofiber and associated stem cell was isolated from a MyoD^−/−;Dll1^luc animal. Quantification of the oscillatory stability (power of the Fast Fourier transformation) and the oscillatory period in control and MyoD^−/− cells (right); each point represents data obtained from a single imaged cell; n = 23 experiments. b Bioluminescence signals from Dll1-luciferase observed in a single Hes1 mutant muscle stem cell associated with a myofiber obtained from a TxHes1;Dll1^luc animal and quantification of this bioluminescence signal (left). Quantification of the oscillatory stability (power of the Fast Fourier transformation) in control and Hes1 mutant cells (right); n = 8 experiments. c Bioluminescence signals from Dll1-luciferase observed in a single muscle stem cell associated with a myofiber treated with siHes1 RNA and quantification of this bioluminescence signal. The myofiber and associated muscle stem cell were isolated from a Dll1^luc animal. d Bioluminescence signals from Dll1-luciferase observed in a single muscle stem cell associated with a fiber and quantification of this bioluminescence signal (left); the fiber and associated stem cell were derived from a MyoD^−/−;Dll1^luc animal and treated with siHes1 RNA. Quantification of the oscillatory stability (power of the Fast Fourier transformation) in siRNA control (n = 5 experiments) and siHes1 RNA (n = 9 experiments) treated wild-type cells, and siHes1 RNA treated MyoD^−/− (n = 6 experiments) mutant cells (right). In the box plot, center lines show the medians; box limits indicate the 25th and 75th percentiles; whiskers extend 1.5 times the interquartile range. Scale bars, 15 μm. Exact p values are indicated, ns indicates P > 0.05, unpaired two-sided t-test.