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. 2021 Jan 27;10(2):243. doi: 10.3390/cells10020243

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Fold-change (2^ΔΔCt) in transcript expression of components of the renin-angiotensin system (RAS): angiotensinogen (AGT), renin, PRR, ACE, ACE2, AT₁R, and AT₂R, as determined by RT-qPCR in four metastatic head and neck cutaneous squamous cell carcinoma (mHNcSCC) tissue samples (A) and four mHNcSCC-derived primary cell lines (B). CT values were normalized to the reference genes GAPDH and PUM1 to calculate ΔCT, and expression compared to that of UHR (y = 1). Error bars represent 95% confidence intervals of the mean. Graphs are shown with log2 scale.