Table 2.
Proportion of PCR mix.
| Component | Volume per 10 μL Reaction |
|---|---|
| SsoAdvanced™ Universal SYBR® Green Supermix | 5 μL |
| Forward and reverse primers | 1 μL (0.8 μM) |
| DNA template | 2 μL (0.04–0.015 × 10−4) |
| Nuclease-free water | 2 μL |
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