Skip to main content
. 2021 Jan 26;11(2):163. doi: 10.3390/biom11020163

Figure 1.

Figure 1

Structural analysis of the framework of VHH A10. Potential camelid residues to be mutated (yellow) and already humanized residues (pink) in the sites formed by the (a) framework 1-3 (FW1-3), and (b) framework 2-4 (FW2-4). In the center, camelid residues of A10 are compared with the model VH residues [29,30,31]. The key hallmarks FERF are highlighted by a red rectangle. (c) Sequence alignment of the frameworks of A10, the reference human VH (sVH) [29], a universal VHH framework (uVHH) [30], and the most humanized VHH sequence available (hVHH) [9]. Crosses indicate the FERF/VGLW hallmarks, while triangles show the already humanized residues of A10.