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Characterization of A10 mutants: A10 wt. and its mutants were purified first by IMAC and then by SEC and the protein yields were calculated after each step. Only the proteins eluted as a monomer were considered after SEC.
| Constructs | Yield IMAC (mg/L) | Yield SEC (mg/L) | Aggregation State (According to SEC Profile) |
|---|---|---|---|
| A10 | 12.6 | 8.9 | monodispersed |
| A10-HLL | 2.0 | 0 | polydispersed |
| A10C | 7.6 | 4.9 | monodispersed |
| A10VI | 4.5 | 0.6 | polydispersed |
