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. 2021 Jan 25;10(2):230. doi: 10.3390/plants10020230

Table 1.

The results of the analysis of the modified extracts of motherwort by HPLC.

Component The Quantitative Content in the Extract, mg/100 g, x¯±Δx¯
Extract with the Motherwort Tincture With Lysine With Aspartic Acid With Leucine With Alanine With Phenylalanine With Glycine With Valine With Histidine With Glutamic Acid With Arginine With Cysteine With Methionine
Derivatives
of caffeic acid-1
1.79 ± 0.04 # 2.37 ± 0.08 1.86 ± 0.03 1.72 ± 0.04 1.89 ± 0.03 2.17 ± 0.05 1.93 ± 0.02 2.47 ± 0.05 2.52 ± 0.04 1.67 ± 0.03 1.84 ± 0.02 1.73 ± 0.03 1.61 ± 0.02
Derivatives
of caffeic acid-2
2.36 ± 0.07 * 2.17 ± 0.04 2.63 ± 0.06 1.94 ± 0.02 2.52 ± 0.04 2.85 ± 0.04 2.35 ± 0.06 2.18 ± 0.07 2.69 ± 0.03 2.02 ± 0.02 2.47 ± 0.05 2.38 ± 0.05 2.13 ± 0.08
Chlorogenic acid 76.87 ± 0.57 # 65.88 ± 0.87 71.3.8 ± 0.87 78.79 ± 0.93 76.38 ± 0.76 69.74 ± 0.77 75.69 ± 0.91 79.84 ± 0.84 75.27 ± 0.99 80.16 ± 0.74 76.19 ± 0.95 75.97 ± 0.69 74.84 ± 0.97
Caffeic acid 5.58 ± 0.11 * 5.08 ± 0.17 6.15 ± 0.18 6.24 ± 0.27 5.69 ± 0.13 5.18 ± 0.15 5.82 ± 0.14 5.93 ± 0.09 5.57 ± 0.09 4.98 ± 0.06 5.29 ± 0.16 5.14 ± 0.17 5.37 ± 0.41
Derivatives
of caffeic acid-3
3.68 ± 0.08 # 3.39 ± 0.05 3.78 ± 0.11 3.66 ± 0.09 3.32 ± 0.08 3.79 ± 0.11 3.08 ± 0.06 3.26 ± 0.12 3.68 ± 0.05 3.58 ± 0.06 2.97 ± 0.07 3.16 ± 0.14 3.05 ± 0.21
Derivatives
of caffeic acid-4
14.4 ± 0.2 # 11.8 ± 0.1 13.7 ± 0.2 12.9 ± 0.2 8.9 ± 0.1 13.2 ± 0.2 15.8 ± 0.2 11.9 ± 0.2 16.4 ± 0.4 11.6 ± 0.2 13.2 ± 0.4 15.7 ± 0.4 7.5 ± 0.2
Derivatives
of caffeic acid-5
1.27 ± 0.02 # 0.98 ± 0.01 1.04 ± 0.01 0.87 ± 0.01 nd 1.21 ± 0.02 0.95 ± 0.01 0.93 ± 0.01 1.13 ± 0.03 1.23 ± 0.03 1.17 ± 0.02 nd 0.87 ± 0.01
Rutin 1.20 ± 0.01 # 1.07 ± 0.02 1.16 ± 0.01 1.27 ± 0.01 1.31 ± 0.02 0.98 ± 0.01 1.17 ± 0.01 1.26 ± 0.01 1.17 ± 0.01 1.24 ± 0.01 1.18 ± 0.02 0.95 ± 0.01 1.01 ± 0.01
Ellagic acid 41.65 ± 0.97 ** 40.87 ± 0.67 37.93 ± 0.73 40.10 ± 0.57 37.69 ± 0.83 42.38 ± 0.55 41.31 ± 0.63 40.87 ± 0.67 42.16 ± 0.29 39.17 ± 0.62 40.38 ± 0.37 40.29 ± 0.91 39.79 ± 0.72
Hyperoside 2.10 ± 0.06 * 2.28 ± 0.10 1.87 ± 0.04 2.04 ± 0.06 2.18 ± 0.04 1.98 ± 0.03 2.09 ± 0.04 2.18 ± 0.05 2.39 ± 0.06 2.18 ± 0.03 2.08 ± 0.06 2.12 ± 0.03 2.07 ± 0.03
Rosmarinic acid 1.72 ± 0.03 *** 1.54 ± 0.04 1.68 ± 0.07 2.01 ± 0.03 2.21 ± 0.05 1.81 ± 0.04 1.75 ± 0.02 1.61 ± 0.04 1.58 ± 0.02 1.52 ± 0.03 1.48 ± 0.02 1.63 ± 0.02 1.54 ± 0.01
Derivatives
of caffeic acid-6
0.97 ± 0.01 # 0.86 ± 0.01 nd 0.59 ± 0.03 0.43 ± 0.01 nd 0.76 ± 0.01 0.86 ± 0.01 0.70 ± 0.01 0.92 ± 0.01 nd 0.58 ± 0.02 0.43 ± 0.01
Derivatives
of caffeic acid-7
0.47 ± 0.01 # 0.41 ± 0.01 nd nd 0.39 ± 0.01 nd 0.35 ± 0.01 nd 0.37 ± 0.01 0.48 ± 0.01 nd nd 0.51 ± 0.01
Catechin 6.25 ± 0.17 ** 5.73 ± 0.12 6.38 ± 0.07 6.02 ± 0.07 5.87 ± 0.07 5.92 ± 0.11 6.01 ± 0.09 6.24 ± 0.17 6.52 ± 0.16 6.08 ± 0.12 5.92 ± 0.31 5.86 ± 0.63 6.17 ± 0.27
Methoxy apigenin 3.95 ± 0.07 # 3.48 ± 0.09 3.35 ± 0.07 4.01 ± 0.07 3.75 ± 0.12 4.06 ± 0.17 3.86 ± 0.13 3.39 ± 0.09 3.52 ± 0.08 3.68 ± 0.07 4.12 ± 0.24 3.86 ± 0.41 3.73 ± 0.21
The amount of flavonoids 13.50 12.56 12.76 13.34 13.11 12.94 13.13 13.07 13.60 13.18 13.30 12.79 1298
The amount of hydroxycinnamic acids 109.11 94.48 102.22 108.72 101.73 99.95 108.48 108.98 109.91 108.16 104.61 106.29 97.85
The amount of identified substances 164.26 147.91 152.91 162.16 152.53 155.27 162.92 162.92 165.67 160.51 158.29 159.37 150.62

According to the t-test, the differences in content of biologically active substances (BAS) in extracts with and without amino acids were considered to be # extremely statistically significant (p < 0.0001), * not statistically significant (p = 0.34–0.97), ** very statistically significant (p = 0.0004–0.015), or *** not quite statistically significant (p = 0.08); nd—not detected.