Table 3.

Variants of the ProBi scaffold sorted by their affinities to IL-10. The table shows affinities, response amplitude during microscale thermophoresis (MST) measurements, number of replicates of the MST measurements, and melting temperatures (Tm) measured by the nanoDSF method. Not measurable indicates that the affinity is so low that it falls beyond the dynamic range of the MST technique.

ProBi Variant (Buffer)	Affinity	Response Amplitude	Replicates	Tm
WT (Tris)	Not measurable	Not measurable	3	66 °C
F5 (Tris)	6 nM	14	4	51 °C
F5 (Hepes)	15 nM	27	3	N/A
G3 (Tris)	208 nM	12	3	58 °C
G3 (Hepes)	364 nM	27	3	N/A
A2 (Tris)	>1 µM	N/A	2	48 °C