Figure 7.

Tofacitinib treatment reduces T cell numbers and partially restores mTOR and JAK/STAT signaling pathways in villin-Cre Hdac1 and Hdac2 deficient mice. Jejunal tissue sections, from wild-type (Ctrl) as well as villin-Cre Hdac1^−/−; Hdac2^−/− mice (Hdac1/2 ΔIEC) treated without or with Tofacitinib (DMSO or TOFA) for five days, were stained with an antibody against CD3 for T cells. T cells are marked by black arrows (A). Average number of CD3+ cells around crypts is shown in (B) Results represent the mean ± SEM (* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.005). Jejunal tissue sections were stained with an antibody against STAT3 (C) and against phospho-S6 ribosomal protein (D). Magnification: 10×.