Figure 6.

Controlled-batch fermentations of selected Ustilaginaceae candidates. OD₆₀₀ of (A) U. maydis #2229, (B) U. rabenhorstiana #2708, and (C) U. cynodontis #2705. Itaconate production is shown for (D) U. maydis #2229, (E) U. rabenhorstiana #2708, and (F) U. cynodontis #2705 during fermentation in a bioreactor containing MTM medium (0.8 g L⁻¹ NH₄Cl, 30 °C, 80% DOT, at pH 6.5). Ustilaginaceae candidates using acetate as a co-substrate are shown in orange, and formate co-substrate cultivations are shown in green (6.25 g L⁻¹). Respective glucose references (50 g L⁻¹) are shown in blue.