Figure 7.

Controlled-batch fermentations of U. maydis #2229 increasing glucose concentration to 200 g L⁻¹. (A) Bioreactor experiments using 200 g L⁻¹ glucose, (B) with 200 g L⁻¹ glucose and 25 g L⁻¹ acetate. Cultivations were performed in MTM medium (0.8 g L⁻¹ NH₄Cl, 30 °C, 80% DOT, at pH 6.5). (C) Overview of itaconate production of both experiments. Cultivations using acetate as a co-substrate are shown in orange (Y_P/S = 0.25 ± 0.00 [g_ITA/g_c-source]), and respective glucose references are shown in blue (Y_P/S = 0.17 ± 0.00 [g_ITA/g_c-source]). Error bars indicate the standard error of the mean (n = 3).