Skip to main content
. 2021 Jan 21;13(2):136. doi: 10.3390/pharmaceutics13020136

Figure 6.

Figure 6

(A) Amount of ALP production in MC3T3-E1 cells after treatment with free BMP-2, heparin/BMP-2, and S-PRX/BMP-2 for 72 h. The concentrations of BMP-2 and the SPE-PRXs were 100 ng/mL and 1,000 μg/mL, respectively. The data are expressed as the mean ± S.D. (n = 3, *** p < 0.005, **** p < 0.001). (B) Alizarin red stained images for MC3T3-E1 cells after treatment with free BMP-2, heparin/BMP-2, and S-PRX/BMP-2 for 21 days. (C) X-ray μ-CT images of the mouse calvarial defect treated with free BMP-2, heparin/BMP-2, and S-PRX/BMP-2 complexes embedded into collagen sponges. The concentrations of BMP-2 and S-PRX were 100 ng/mouse and 1000 μg/mouse, respectively. The amount of implanted heparin was 100 μg/mouse. Reproduced with permission from [127] © 2015 Wiley and [128] © 2017 Wiley.