Table 3.
Primer sequences used for quantitative RT-PCR.
| Gene | Sequence (5′–3′) | Product Size (bp) | Accession No. |
|---|---|---|---|
| SGLT1 | F: GCAACAGCAAAGAGGAGCGTAT | 95 | NM_001164021.1 |
| R: GCCACAAAACAGGTCATAGGTC | |||
| SLC7A1 | F: CTTTCTACCCGCGGTCTCC | 150 | NM_001012613.1 |
| R: TGCTGAGCGAATCTGCTGTA | |||
| ZO-1 | F: CAGCCCCCGTACATGGAGA | 114 | XM_005659811 |
| R: GCGCAGACGGTGTTCATAGTT | |||
| Occludin | F: CTACTCGTCCAACGGGAAAG | 158 | NM_001163647.2 |
| R: ACGCCTCCAAGTTACCACTG | |||
| TNF-α | F: ACCACGCTCTTCTGCCT | 121 | NM_214022.1 |
| R: GGCTTATCTGAGGTTTG | |||
| IL-8 | F: AGTGGACCCCACTGTGAAAA | 102 | X61151.1 |
| R: TACAACCTTCTTCTGCACCCA | |||
| TGF-β | F: AGGACCTGGGCTGGAAGTG | 119 | NM_214015.1 |
| R: GGGCCCCAGGCAGAAAT | |||
| IL-1β | F: TCTGCCCTGTACCCCAACTG | 112 | NM_214055.1 |
| R: CCAGGAAGACGGGCTTTTG | |||
| β-actin | F: CCACGCCCTTTCTCACTTGT | 114 | DQ178122 |
| R: CACCCACAGCACCTTATGCT |
SGLT1, sodium-glucose cotransporter 1; SLC7A1, solute carrier family 7 member 1; ZO-1, zonula occluden-1; TNF-α, tumor necrosis factor-α; IL-8, interleukin-8; TGF-β, transforming growth factor-β; IL-1β, interleukin-1β.