Table 4.

Primer and probe sequences used for quantitative RT-PCR.

Items	Sequence (5′–3′)	Anneal Temperature (°C)	Product Size (bp)
Total bacteria	F: ACTCCTACGGGAGGCAGCAG R: ATTACCGCGGCTGCTGG	60.0	200
Lactobacillus	F: GAGGCAGCAGTAGGGAATCTTC R: CAACAGTTACTCTGACACCCGTTCTTC P: AAGAAGGGTTTCGGCTCGTAAAACTCTGTT	57.5	126
Bifidobacterium	F: CGCGTCCGGTGTGAAAG R: CTTCCCGATATCTACACATTCCA P: ATTCCACCGTTACACCGGAA	59.5	121
Bacillus	F: GCAACGAGCGCAACCCTTGA R: TCATCCCCACCTTCCTCCGGT P: CGGTTTGTCACCGGCAGTCACCT	60.0	92
Escherichia coli	F: CATGCCGCGTGTATGAAGAA R: CGGGTAACGTCAATGAGCAAA P: AGGTATTAACTTTACTCCCTTCCTC	58.8	96