Table 1.
mRNA fold-changes (FC) and standard deviations (SDs) determined by qRT-PCR for SOS response and DNA repair genes in aerobic Mtb H37Rv cells treated with 2 × MIC (0.25 μg/mL) of MX for 16 h, compared to control cells grown in the absence of the drug F and R: forward and reverse primers, respectively (*), p < 0.02; (**), p < 0.002; (-), not significant.
| Category | Rv No. | Gene | FC ± SD | p | F | R |
|---|---|---|---|---|---|---|
| SOS response | Rv1378c | - | 43.85 ± 8.93 | ** | ACGCCCACCGGGATGTACTA | GAGGGCGACACCGATTCTGG |
| Rv0336 | - | 6.25 ± 0.93 | ** | TGATCACCGCCGAACTGGTG | CCAGCGACACGTCAGATCCC | |
| Rv0515 | - | 6.25 ± 0.93 | ** | TGATCACCGCCGAACTGGTG | CCAGCGACACGTCAGATCCC | |
| Rv1000c | - | 8.09 ± 1.41 | ** | ACATCTACGGCGGCGAACTG | CCGTCGCGGTAGTAGCACAG | |
| Rv2719c | - | 40.58 ± 5.61 | ** | CCGCGGCGATTACTCTCTGG | GGACCGCCACGTCATACAGG | |
| Rv3395c | - | 70.31 ± 4.87 | ** | GGACGGTGGGAGTGCTGTC | ACCGATGCCACCATGCTCAG | |
| Rv0427c | xthA | 4.49 ± 0.18 | ** | ATCGCACTGATGGGCGACTG | AATTGCGCGTCGACAATGGC | |
| Rv3260c | whiB2 | 0.32 ± 0.02 | ** | GGAAGCCACCGACCAATG | CCAGGGCGTACTCCAGAC | |
| DNA repair | Rv0054 | ssb | 15.58 ± 3.70 | * | CGTCAGACCGGCGAATGGAA | TCGATGACGGTGCGCTTCTC |
| Rv1638 | uvrA | 2.55 ± 1.50 | - | CCCGAGATTCTGGCGGTGAC | GCGACCGGATCTCCTTGAGC | |
| Rv2594c | ruvC | 9.90 ± 1.23 | ** | ACGTGGTGTCGACGTGCATT | GACCATCGCGGTGACCTGAG | |
| Rv3585 | radA | 12.18 ± 1.41 | ** | CGGTATCGTTCCCGGTTCGG | CTGACCGGCGGATTCCTCAC | |
| Rv2821c | - | 0.85 ± 0.21 | - | AGCAGGCTGCCGATGATTCC | GAGCTTCGTGTCGCGGAAGA |