Skip to main content
. 2021 Jan 30;9(2):289. doi: 10.3390/microorganisms9020289

Figure 2.

Figure 2

Schematic genetic organization and co-transcription verification of polysaccharide biosynthesis gene clusters. (A) Schematic genetic organization of two polysaccharide biosynthesis gene clusters in P. polymyxa WLY78. The pep-1 cluster comprises 12 putative genes and the pep-2 gene cluster contains 17 putative genes. The interval region between the pep-1 cluster and the pep-1 cluster is ~2 kb. The brace indicates the knockout genes. Different colors indicate different functions of genes. Black rows indicate primers used for RT-PCR. (B) Confirmation of each of the pep-1 cluster and the pep-2 cluster being organized in an operon by RT-PCR. Result of RT-PCR reactions with RNA from P. polymyxa WLY78 in agarose gel. The numbers on the top of the gel correspond to the numbers marked schematically in the outline given above. (+), genomic DNA served as a positive control. (−), RNA template was used as the negative control.