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. 2021 Jan 30;9(2):289. doi: 10.3390/microorganisms9020289

Figure 5.

Figure 5

Effect of biofilm formation defect on nitrogenase activity under aerobic conditions. (A) The influence of biofilm on the nitrogenase activity under anaerobic condition in the planktonic state or under aerobic conditions in the biofilm state. (B) qRT-PCR analysis of the relative mRNA levels of the nifHDK genes in the WT and ∆pep-2 strains under anaerobic condition in the planktonic state. (C) qRT-PCR analysis of the relative mRNA levels of the nifHDK genes in the WT and ∆pep-2 strains under aerobic condition in the biofilm state. Relative nitrogenase activity and relative mRNA levels of each sample were normalized to those of the WT sample. Results are representative of at least three independent experiments. Error bars indicate SD. Ns indicates nonsignificant. ** indicates p < 0.01. *** indicates p < 0.001.