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. 2021 Jan 30;9(2):289. doi: 10.3390/microorganisms9020289

Figure 6.

Figure 6

Effects of nitrogen and oxygen on nitrogenase activity in biofilm. (A) Effect of different O2 concentrations on biofilm formation. (B) Effect of O2 level on the nitrogenase activity of P. polymyxa WLY78 in the biofilm state. (C) Effect of different concentrations of NH4Cl or glutamate on nitrogenase activity of P. polymyxa WLY78 in the biofilm state. Relative nitrogenase activity of each sample was normalized to that of sample under the anaerobic environment in the biofilm state. Results are representative of at least three independent experiments. Error bars indicate SD. ** indicates p < 0.01. *** indicates p < 0.001.