Table 1.
The predicted functions of the 29 genes within the pep-1 and pep-2 clusters in P. polymyxa WLY78.
| Predicted Gene Products | Length [aa] | Predicted Function | Predicted Protein Family | Identity with Those of P. polymyxa DSM 365 |
|---|---|---|---|---|
| pep-2 gene cluster | ||||
| PepA (GM004746) |
247 | Capsular biosynthesis protein | YveK | 100% |
| PepB (GM004745) |
212 | tyrosine protein kinase | P-loop_NTPase | 100% |
| GalU (GM004744) |
300 | UTP-glucose-1-phosphate uridilytransferase | Glyco_tranf_GTA_type (Glycosyltransferase family A) |
100% |
| PepC (GM004743) |
232 | undecaprenyl-phosphate galactose phosphotransferase |
Bac_transf (Bacterial sugar transferase) | 99.57% |
| PepD (GM004742) |
311 | undecaprenyl-phosphate galactose phosphotransferase | Glyco_tranf_GTA_type | 99.36% |
| PepE (GM004741) |
445 | polysaccharide polymerase | Wzy_C | 99.78% |
| PepF (GM004740) |
251 | UDP-N-acetyl-D-mannosaminuronic acid transferase |
Glycosyltransferase WecG/TagA | 100% |
| PepG (GM004739) |
480 | polysaccharide polymerase | Wzy_C | 99.38% |
| PepH (GM004738) |
472 | flippase | MATE (Multidrug and toxic compound extrusion) like | 99.79% |
| PepI (GM004737) |
276 | glycosyltransferase GT2 | Beta4 Glucosyltransferase | 99.64% |
| PepJ (GM004736) |
395 | glycosyltransferase GT4 | Glycosyltransferase_GTB-type | 100% |
| PepK (GM004735) |
382 | glycosyltransferase GT1 | Glycosyltransferase_GTB-type | 100% |
| PepL (GM004734) |
354 | glycosyltransferase GT4 | Glycosyltransferase_GTB-type | 100% |
| UgdH1 (GM004733) |
445 | UDP-glucose 6-dehydrogenase | NADB[NAD(P)H/NAD(P)(+) binding]_Rossmann | 100% |
| ManC (GM004732) |
458 | mannose-1-phosphate guanylyltransferase | Glyco_tranf_GTA_type | 99.56% |
| PepM (GM004731) |
535 | glycoside hydrolase | Glycosyl hydrolase family 26 | 96.45% |
| PepN (GM004730) |
741 | glycoside hydrolase | CRISPR/Cas system-associated protein Cas4 | 97.98% |
| pep-1 gene cluster | ||||
| PepO (GM004727) |
249 | polysaccharide chain length regulation protein |
YveK | 97.59% |
| PepP (GM004726) |
228 | tyrosine protein kinase | P-loop_NTPase | 98.68% |
| PepQ (GM004725) |
192 | undecaprenyl-phosphate glucose phosphotransferase |
Bac_transf | 98.96% |
| Fcl (GM004724) |
312 | GDP-L-fucose synthase | NADB_Rossmann | 98.40% |
| Gmd (GM004723) |
329 | GDP-mannose 4,6-dehydratase | NADB_Rossmann | 99.39% |
| PepR (GM004722) |
447 | putative flippase | MATE_like | 99.78% |
| PepS (GM004721) |
420 | putative flippase | No domains | 100% |
| PepT (GM004719) |
416 | glycosyltransferase GT1 | Glycosyltransferase_GTB-type | 96.88% |
| PepU (GM004718) |
405 | glycosyltransferase GT1 | Glycosyltransferase_GTB-type | 97.78% |
| PepV (GM004717) |
269 | UDP-N-acetyl-D-mannosaminuronic acid transferase | Glycosyltransferase WecG/TagA | 95.54% |
| UgdH2 (GM004716) |
456 | UDP-glucose 6-dehydrogenase | NADB_Rossmann | 99.12% |
| LytR (GM004715) |
325 | transcriptional regulator | LytR_cpsA_psr | - |