Table 1.
Real-time qPCR-based overexpression data (fold-change, relative normalized expression—RNE) of the created mutant strains. Data were normalized to the CLIB214 wild type strain. Relative transcription levels were determined using the housekeeping gene CpTUB4 (CPAR2_500510) as an internal control. Measurements were performed in three statistical parallels. The 2−∆∆Cq comparison method was used with Bio-Rad CFX Manager software. (Cp—Candida parapsilosis; Ca—C. albicans; Sc—Saccharomyces cerevisiae).
| Gene Name | Ortholog Name | RNE | Gene Name | Ortholog Name | RNE |
|---|---|---|---|---|---|
| CPAR2_100460 | CpHBT4 | 122.5 ± 17.7 | CPAR2_302400 | ScMGT1 | 424.4 ± 90.5 |
| CPAR2_100470 | CpHBT3 | 18.6 ± 0.9 | CPAR2_303240 | ScPFA5 | 44.7 ± 2.8 |
| CPAR2_100540 | CaHAP5 | 7.6 ± 0.9 | CPAR2_303730 | ScTHR1 | 66.1 ± 26 |
| CPAR2_104420 | ScLTE1 | 378.5 ± 22.1 | CPAR2_400270 | ScHIT1 | 31.6 ± 2.3 |
| CPAR2_105250 | CaPHO85 | 44.8 ± 3.5 | CPAR2_406400 | ScRPA12 | 41.7 ± 7.3 |
| CPAR2_107020 | CaHHF1 | 118.2 ± 11.0 | CPAR2_500180 | ScKIN3 | 675.7 ± 89.3 |
| CPAR2_107240 | CaKTR4/MNT4 | 70.4 ± 12.4 | CPAR2_500360 | ScAMS1 | 37.2 ± 2.6 |
| CPAR2_108840 | ScSPS1 | 50.5 ± 9.6 | CPAR2_501400 | CaCWH41 | 90.0 ± 11.2 |
| CPAR2_109520 | CaTUP1 | 19.1 ± 4.5 | CPAR2_503290 | CaRAD53 | 218.4 ± 25.9 |
| CPAR2_200040 | - | 212.2 ± 17.3 | CPAR2_503760 | ScTMA46 | 7.3 ± 0.7 |
| CPAR2_200390 | CaSPT3 | 9.0 ± 0.6 | CPAR2_602370 | ScRAD18 | 17.3 ± 2.1 |
| CPAR2_201920 | ScMNT2 | 7.3 ± 1.1 | CPAR2_602820 | CaFCA1 | 28.4 ± 8.4 |
| CPAR2_204840 | CpHBT2 | 8.9 ± 1.8 | CPAR2_602840 | ScTFB4 | 176.7 ± 30.6 |
| CPAR2_205060 | CaCDC28 | 30.6 ± 5.6 | CPAR2_700550 | ScRSC8 | 4.5 ± 0.5 |
| CPAR2_208600 | CaCPH1 | 44.5 ± 3.6 | CPAR2_703840 | ScSLM5 | 52.5 ± 8.4 |
| CPAR2_209240 | CaCDC19/PYK | 2.6 ± 0.0 | CPAR2_804030 | ScGSC2 | 134.6 ± 29.9 |
| CPAR2_209520 | CaMKK2 | 7.8 ± 1.3 | CPAR2_805930 | CaTEC1 | 34.5 ± 6.9 |
| CPAR2_300080 | ScADK2 | 134.8 ± 7.3 | CPAR2_806950 | ScNOB1 | 65.7 ± 9.9 |
| CPAR2_301360 | CaPMR1 | 38.9 ± 3.1 |