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. 2021 Jan 29;10(2):135. doi: 10.3390/pathogens10020135

Figure 3.

Figure 3

PCR demonstrating the integration of Bm86Ch and eGFP-BSD into the B. bovis genome at the expected the ef-1α locus in the parasite line B. bovis/Bm86/eGFP: the PCRs were performed using 1) B. bovis S74-T3Bo gDNA (wild type strain), 2) B. bovis/Bm86/eGFP gDNA, 3) pBluescript plasmid DNA, and 4) pEf/Bm86/eGFP plasmid DNA. The individual panels show the PCR amplicons obtained with primers MSA-1 F/R (a), eGFP F/BSD R (b), MSA-1sig F/Bm86 R (c), eGFP F/UPS-Ef-probe R (d), and Ef-A-probe F/Bm86 (e).