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. 2021 Jan 29;10(2):135. doi: 10.3390/pathogens10020135

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of Bm86Ch and eGFP by B. bovis/Bm86/eGFP demonstrated by Western blot analysis: numbers 1 to 5 indicate the lysate of B. bovis S74-T3Bo, the lysate of transfected B. bovis/Bm86/eGFP, the recombinant eGFP-BSD, the recombinant Bm86, and the R. microplus midgut, respectively. (A) Monoclonal antibody (Ab) anti-MSA-1 Babb35. (B) Rabbit polyclonal Ab anti-GFP. (C) Rabbit polyclonal Ab anti-Bm86. (D) Pre-immune rabbit serum. The protein molecular weight marker (M) is shown on the right side of each immunoblot.