Table 1.
Toxoplasma gondii endocytic proteins.
| GeneID | Protein Name |
Domain/Complex | Known or Prospective Function | Phenotype Score [87] |
|---|---|---|---|---|
| TGME49_290950 | Clathrin | Heavy chain | Interacts with AP-1 mu1 at TGN [8], promotes microneme and rhoptry trafficking at the TGN [81] | −4.77 |
| TGME49_221940 | AP2 | Alpha subunit | Clathrin adaptor for endocytosis? [88] | −4.32 |
| TGME49_240870 | Beta2 subunit | −2.83 | ||
| TGME49_230920 | Mu2 subunit | −1.60 | ||
| TGME49_313450 | sigma-1 subunit | −2.88 | ||
| TGME49_320760 | Hypothetical | BAR/IRSp53 and MIM homology domain (IMD)-like domain | Promote membrane curvature? | 0.56 |
| TGME49_259720 | Hypothetical | BAR/IMD-like domain | Promote membrane curvature? | −4.55 |
| TGME49_216030 | Hypothetical | ENTH/ Vps-27, Hrs and STAM (VHS) domain | Promote membrane curvature, clathrin adaptor protein? | −0.67 |
| TGME49_227800 | TgEPS15 | Eps15 homology domain | Clathrin-mediated endocytosis nucleation/scaffold protein? | −3.19 |
| TGME49_214180 | TgEpsL | ENTH domain | Interacts with TgAP-1 at TGN but likely not AP-2, formation of clathrin coated vesicles in secretory trafficking [8] | −0.52 |
| TGME49_213370 | Frm3 | Actin binding | Actin nucleation during endocytosis? [89] | −2.79 |
| TGME49_267045 | Hypothetical | Ras superfamily/Rho GTPase | Regulation of actin during endocytosis? | 0.81 |
| TGME49_249170 | Hypothetical | Ras superfamily/Rho GTPase | Regulation of actin during endocytosis? | −0.79 |
| TGME49_233300 | Hypothetical | Rho GTPase activating protein (RhoGAP) | Regulation of actin during endocytosis? | 1.52 |
| TGME49_321620 | DrpB | GTPase | Microneme and rhoptry trafficking at the TGN | −4.91 |
| TGME49_270690 | DrpC | GTPase | Mitochondrial maintenance? [81] | −4.54 |
| TGME49_267810 | Rab5A | GTPase | Microneme and rhoptry trafficking at the TGN/ELCs [5] | −4.48 |
| TGME49_207460 | Rab5B | GTPase | Undefined [5], recycling to plasma membrane? | 0.55/−1.35 |
| TGME49_219720 | Rab5C | GTPase | Microneme and rhoptry trafficking at the TGN/ELCs [5] | −4.24 |
| TGME49_248880 | Rab7 | GTPase | Undefined [5], fusion with the lysosome? | −2.67 |
| TGME49_230220 | Vps11 | CORVET/HOPS | Exocytic trafficking to the micronemes and rhoptries [6], biogenesis of the VAC [6], endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −4.09 |
| TGME49_320670 | Vps16 | CORVET/HOPS | Exocytic trafficking to the micronemes and rhoptries? Endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −4.82 |
| TGME49_289730 | Vps18 | CORVET/HOPS | Exocytic trafficking to the micronemes and rhoptries [6], endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −3.24 |
| TGME49_295000 | Vps33 | CORVET/HOPS | Exocytic trafficking to the micronemes and rhoptries? Endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −4.78 |
| TGME49_224270 | Vps41 | HOPS | Exocytic trafficking to the micronemes and rhoptries? Endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −3.75 |
| TGME49_315530 | Vps39 | HOPS | Exocytic trafficking to the micronemes and rhoptries [6], endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −4.18 |
| TGME49_291120 | Mon1 | SAND1/Mon1-Ccz1 | Rab7 GEF? Exocytic trafficking to the micronemes and rhoptries [6], endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −4.42 |
| TGME49_207960 | Ccz1 | SAND1/Mon1-Ccz1 | Rab7Gef? Exocytic trafficking to the micronemes and rhoptries? Endocytic trafficking through Rab5 and Rab7 compartments to the VAC? | −3.96 |
| TGME49_271060 | Vps45 | Sec1 family protein | Vesicular trafficking of endocytosed material to the VAC [90] | −5.04 |
| TGME49_224710 | VSR1 | PA domain | Undefined, cargo recognition for sorting to the VAC? | −0.90 |
| TGME49_312860 | VSR2 | PA domain | Undefined cargo recognition for sorting to the VAC? | −0.09 |