Table 2.
Summary of the main conclusions of uncoating studies with respect to the labeling strategy used. (h.p.i. hours post infection)
| Capsid Labeling Strategy | Cells | Timing | Conclusions | Ref. |
|---|---|---|---|---|
| IF | CHO A745 HeLa |
6 h.p.i. 7.5 h.p.i. |
Late uncoating CA present in the nucleus |
[29] |
| MDMs | 48 h.p.i. | No capsid loss between cytoplasm and nucleus | [75] | |
| SupT1-R5 (CD+T cell line) | 0.5 h.p.i | Cytoplasmic loss of ~50% of CA molecules | [19] | |
| SupT1-R5 (CD+T cell line) | 5.5 h.p.i. | Uncoating at NPC | ||
| MDMs | 48 h.p.i. | No capsid loss between cytoplasm and nucleus | ||
| Cyp-DsRed | HeLa derived and MDMs | <1 h.p.i. | Early cytoplasmic uncoating results in proteolytic degradation of the viral complex | [13,17,73] |
| HeLa derived MDMs |
~4 h.p.i. ~11.5 h.p.i. |
Uncoating at the NPC with a fraction of capsid molecules entering into the nucleus with PICs | ||
| CA-eGFP | HeLa | 6 h.p.i | Uncoating at the NE CA nuclear entry independent of PICs |
[28] |
| GFP-CA | HeLa | 10.5 h.p.i. | Intact capsid enters the nucleus | [18] |