Table 1.
Evidence for a mitochondrial localization of Sideroflexins.
| SFXN | Model | Localization | Experiment | Reference |
|---|---|---|---|---|
| SFXN1 | Mouse | IMM | Co-fractionation | Fleming et al. 2001 [1] |
| Human cells (Jurkat, K562) |
Immunoblot on affinity-purified mitochondria STED (co-localization of Flag-SFXN1 and COX4) |
Kory et al. 2018 [8] | ||
| Human cells (MCF7, HT1080), Drosophila | Immunoblot on mitochondrial extracts (fractionation) Confocal microscopy, Proteomics (LC-MS/MS on SFXN1 IP) |
Our unpublished data | ||
| Human cells (HEK) | SILAC-based proteomics coupled LC-MS/MS, carbonate extraction, digitonin fractionation | Acoba et al. 2020 [16] | ||
| SFXN2 | Human cells (HeLa) | OMM or IMM | Confocal microscopy (Tom20 co-localization) | Mon et al. 2018 [9] |
| Human cells (Jurkat, K562) |
Immunoblot on affinity-purified mitochondria | Kory et al. 2018 [8] | ||
| Human cells (HEK) | SILAC-based proteomics coupled LC-MS/MS | Acoba et al. 2020 [16] | ||
| SFXN3 | Rat embryonic brain cells | IMM | Fractionation, Confocal microscopy (co-localization with COX4), TEM | Rivell et al. 2019 [18] |
| Human cells (Jurkat, K562) | Immunoblot on affinity-purified mitochondria | Kory et al. 2018 [8] | ||
| Human cells (HEK) | SILAC-based proteomics coupled LC-MS/MS | Acoba et al. 2020 [16] | ||
| SFXN4 | Human cells (HeLa) | IMM | Fractionation and protease protection assay | Hildick-Smith et al. 2013 [19] |
| Human cells (Jurkat, K562) | Immunoblot on affinity-purified mitochondria | Kory et al. 2018 [8] | ||
| Human cells (HEK) | SILAC-based proteomics coupled LC-MS/MS | Acoba et al. 2020 [16] | ||
| SFXN5 | Human cells (HEK) Mouse astrocytes, human cortex and spinal cord |
SILAC-based proteomics coupled LC-MS/MS Immunocapture of GFP-OMM-tagged mitochondria (MitoTag mice), immunostaining |
Acoba et al. 2020 [16] Fecher et al. 2019 [20] |
IMM: inner mitochondrial membrane, IP: immunoprecipitation, OMM: outer mitochondrial membrane, STED: stimulated emission depletion, TEM: Transmission Electron Microscopy, SILAC: Stable isotope labelling of amino acids, LC-MS/MS: Liquid chromatography and tandem mass spectrometry.