. 2021 Jan 21;9(2):103. doi: 10.3390/biomedicines9020103

Table 3.

Regulation of systemic or cellular iron levels by SFXN.

Protein	Model	Evidence	Methodology	Reference
SFXN1	Mouse	Iron overload in mitochondria of erythrocytes in the flexed-tail mouse	Iron mitochondrial staining	Fleming et al. 2001 [1]
SFXN1	HEK SFXN1 KO cells	Increased mitochondrial iron	ICP-MS	Acoba et al. 2020 [15]
SFXN2	HEK SFXN2 KO cells	Increased mitochondrial iron levels	ICP-MS Mito-FerroGreen staining and confocal microscopy	Mon et al. 2019 [9]
SFXN3	Mouse Sfnx3 KO	Decreased circulating iron levels in male transgenic mice homozygous for the Sfxn3^{tm1b(KOMP)Wtsi} allele	Biochemical assay	The IMPC database ¹
SFXN4	K562 SFXN4 KO cells	Decreased labile iron pool	Indirect biochemical measure based on the dequenching of calcein upon release of iron TEM-EDX	Paul et al. 2019 [31]
SFXN4	K562 SFXN4 KO cells	Increased mitochondrial iron levels		Paul et al. 2019 [31]
SFXN5		no data available	-	-

ICP-MS is for inductively coupled plasma atomic emission—mass spectrometry, TEM-EDX is for Transmission electron microscopy—Energy dispersive X-ray analysis. ¹ website page for SFXN3: https://www.mousephenotype.org/data/genes/MGI:2137679#phenotypesTab.—means no data available.