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Experimental plate designs for intra-plate variability. Lysozyme (L): three 384-well plates were analyzed (run 1, run 2, and run 3), and the concentration series was arranged horizontally (2, 3.5, 5, 7, 8, 10, 12, and 14 mg/mL). Lysozyme batch types: LysSig2243 (blue), +400-mM NaCl (cyan) and LysSig5161 (green), +400-mM NaCl (light green). Control samples: Ac.B: 20-mM acetate buffer, pH 4.5, and NaCl: 400-mM of sodium chloride in acetate buffer, pH 4.5.
