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Experimental plate designs for inter-plate variability. Peptide-12 (P): three 384-well plates were analyzed: PA2-4: A4.5 = acetate buffer, pH 4.5; the concentration series were arranged horizontally; 4 replicates were positioned vertically. (1 = 2 mg/mL, 2 = 4 mg/mL, 3 = 6 mg/mL, 4 = 8 mg/mL, 5 = 10 mg/mL API, and 0 = placebo). Glycerol (orange) and sodium chloride (light orange) were added as tonicity agents, and formulations without tonicity agents were marked in dark orange.
