Table 1.

Effect of activated charcoal (AC) on the plantlet regrowth of encapsulated Castanea sativa EAs subjected to various dehydration times with PVS2 following immersion in LN evaluated 5 weeks after cryostorage, rewarming and plating.

PVS2 (min)	Regrowth a (AC−)		Regrowth a (AC+)
	Shoot Length (mm)	Root Length (mm)	Shoot Length (mm)	Root Length (mm)
0	4.0 b	5.2 b	5.0 c	6.3 c
30	9.2 a	10.2 a	14.5 a	22.8 a
60	6.5 b	8.8 a	8.7 b	9.8 b
90	5.9 b	8.9 a	8.3 b	10.4 b
120	4.9 b	7.8 a	7.9 b	9.5 b

^a Mean of 90 plantlets tested. Data were recorded after 5 weeks of culture following cryopreservation. Statistical analysis in each column was performed by ANOVA. Data followed by different letters are significantly different at p ≤ 0.05 by LSD test.