Table 2.
Primers, annealing temperatures and product size used for Mycobacterium avium subsp. paratuberculosis identification in the present study.
| Primer Name | Primer Sequences | Annealing (°C) | Product Size (bp) | References |
|---|---|---|---|---|
| IS900-F | 5′ CCTTTCTTGAAGGGTGTTCG 3′ | 58 | 548 | [17] |
| IS900-R | 5′ CCACCAGATCGGAACGTC 3′ | |||
| DMC1-529 F | 5′ GCTGTTGGCTGCGTCATGAAG 3′ | 60 | 310 (C-type) | [26] |
| DMC1-531 F | 5′ TCTTATCGGACTTCTTCT GGC 3′ | 162 (S-type) | ||
| DMC1-533 R | 5′ CGGATTGACCTGCGTTTCAC 3′ | |||
| pig-RDA10 P19 | 5′ TAG CGG TCC CGC AGT TTG GC 3′ | 61 | 382 | [27] |
| pig-RDA10 P20 | 5′ TCA AGC CGA ACG AGG TGG TCG 3′ | |||
| pig-RDA20 P21 | 5′ TCG TCC CGT CCC GAT GCT GT 3′ | 560 | ||
| pig-RDA20 P22 | 5′ TGA GTC CTG TCG TGC ATG CG 3′ | |||
| pig-RDA30 P23 | 5′ TGA AGA GCC CGG ACA AGG GG 3′ | 525 | ||
| pig-RDA30 P24 | 5′ TAG GTC TCA GTG GTC CAC CAG C 3′ |