Figure 3.

The expression of vimentin, E-cadherin, cytokeratin, and Epidermal Growth Factor Receptor (EGFR) in untreated and decidualized endometrial stromal cells (ESCs) studied to confirm mesenchymal to epithelial transition (MET). (A) confocal microscopy images demonstrating alterations in vimentin (green), E-cadherin (green) and cytokeratin (red) expression; (B) an example of Western blot technique utilization in the research of expression of vimentin, E-cadherin, cytokeratin, and GAPDH; (C) a graph of gene expression determined using the qPCR technique demonstrating the expression increase in EGFR in decidualized cells and the expression decrease in untreated cells [15].