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. 2021 Jan 28;10(2):251. doi: 10.3390/cells10020251

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Methods and characterization of exosomes extracted from Nsc-SBOs and Sc-SBOs. (A,B) Exosomes were isolated from culture medium of Nsc-SBOs and Sc-SBOs. (C) Exosome size and concentration was measured using NS300 NanoSight (n = 3 patients). (D) Western blotting analysis of exosomal markers in Nsc-SBOs and Sc-SBOs exosomes (images representative of n = 3 patients). (E,F) TEM images of purified exosomes derived from SBOs. The images are representative of exosomes isolated from three different patients. Nsc, nonsclerotic; Sc, sclerotic; SBOs, subchondral bone osteoblasts; Nsc-exo, nonsclerotic SBO derived exosomes; Sc-exo, sclerotic SBO derived exosomes.