Figure 2.

Sc-SBOs secreted exosomes contribute to cartilage degeneration. (A) Coculturing model of ACCs pellets and Nsc/Sc-SBOs exosomes. (B) Proliferation assay of ACCs cocultured with SBOs exosomes in 0/10/20/50 µg/mL (n = 3). (C) Alcian blue staining (top) and immunostaining (bottom) analysis of ACCs pellets cocultured with Nsc/Sc-SBOs exosomes (n = 3 patients). (D) PKH67-marked exosomes staining with ACCs (images are representative of n = 3 patients) and negative control. (E) Real-time PCR analysis of ACCs pellets cocultured with Nsc/Sc-SBO derived exosomes (n = 3), represented as the mean ± SD; *, p < 0.05, **, p < 0.01, ***, p < 0.001, ****, p < 0.0001; ##: Very significant, #: Significant; ns: Not significant