Table 1.
PARP1 target gene-specific sequences and housekeeping genes (HKGs) and their respective FAM-labeled universal probe library (UPL) probes and gene association numbers for real-time PCR.
| Genes | Forward (F) and Reverse (R) Primers Sequences |
UPL Probes | Gene Accession Number |
|---|---|---|---|
| Target Gene | |||
| PARP1 | F: TCTTTGATGTGGAAAGTATGAAGAA R: GGCATCTTCTGAAGGTCGAT |
#22 | NM_001618.3 |
| Housekeeping Genes (HKGs): | |||
| POLR2A | F: TCCGTATTCGCATCATGAAC R: TCATCCATCTTGTCCACCAC |
#69 | NM_000937 |
| PPIA | F: TTCATCTGCACTGCCAAGAC R: CACTTTGCCAAACACCACAT |
#158 | NM_021130 |
| GAPDH | F: TGGTATCGTGGAAGGACTCA R: GCAGGGATGATGTTCTGGAG |
#158 | NM_002046 |