Figure 2.

Detection of TiLV-specific serum neutralizing antibodies induced in the vaccinated tilapia: five serum samples from each group collected pre-vaccination (PV) and at three and six weeks post primary immunization (WPPI) were tested by the serum neutralization test to determine the antibody titers against the virulent isolates TiLV 2017A. A comparison between the vaccinated groups V1, V2, V3, VA1, VA2, and VA3 with the NC or A groups is shown. Differences in the antibody levels between vaccinated and control fish were analyzed by the Student’s t-test. The different numbers of asterisks indicate significant differences in antibody levels at * p < 0.05, ** p < 0.05, *** p < 0.01 and **** p < 0.001.