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. 2021 Jan 25;9(2):86. doi: 10.3390/vaccines9020086

Table 2.

Genes and primer sequences used in the qRT-PCR assays.

Genes Forward (5′-3′) Reverse (5′-3′) Product Size GenBank ID References
β-actin GCGGAATCCACGAAACCACC CTGTCAGCGATGCCAGGGTA 183 bp AB270897.1 [56]
MHC-Ia TTCTCACCAACAATGACGGG AGGGATGATCAGGGAGAAGG 132 bp CP003810.1 [57]
MHC-II AGTGTGGGGAAGTTTGTTGGAT ATGGTGACTGGAGAGAGGCG 156 bp JN967618.1 [57]
TNFα CTCAGAGTCTATGGGAAGCAG GCAAACACGCCAAAGAAGGT 216 bp NM_001279533 [57]
IFN-γ CCAACAACTCAGGCTCGCTA TGCTCATGGTAGCGGTGTTT 100 bp KF294754.1 [56]
CD4 AAGAAACAGATGCGGGAGAGT AGCAGAGGGAACGACAGAGAC 100 bp XM_005455473.3 [58]
IL-1β AACACTGACAGAACAACTGCGAACA TCGCAGTTGTTCTGTCAGTGTTGTT 124 bp XM_019365844 [57]