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. 2021 Jan 31;10(2):277. doi: 10.3390/plants10020277

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Some steps of droplet-vitrification and V cryo-plate cryopreservation procedures of Stevia rebaudiana. Droplet-vitrification: (a) PVS2 drops in aluminum foil; (b) explants placed into drops of PVS2; (c) aluminum foil strip with explants, plunged in cryovial filled with LN; (d) thawing of aluminum foil with explants in washing solution. V cryo-plate: (e) drops of sodium alginate in wells of cryo-plate; (f) explants into wells covered with calcium chloride solution; (g) cryo-plate, after PVS2 treatment, placed in cryovial filled with LN; (h) cryopreserved shoots with DV procedure after first subculture; (i) cryopreserved shoots with V cryo-plate procedure after second subculture.