Table 2.
First subculture after droplet-vitrification procedure: morphological parameters of S. rebaudiana shoots, from control and from cryopreserved apical shoot tips and axillary shoot tips. (Means ± SD).
| Treatment |
1 Shoot Length (cm) |
Shoots/Explants (n°) |
Explants with Shoot (%) |
|||
|---|---|---|---|---|---|---|
| Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
|
| Control | 4.30 ± 0.48 ab | 4.21 ± 0.64 a | 0.96 ± 0.63 a | 0.89 ± 0.73 a | 78.0 a | 68.0 a |
| PVS2 30 min + LN | 4.00 ± 0.39 b | 3.64 ± 0.44 b | 0.84 ± 0.55 a | 0.21 ± 0.42 b | 77.0 a | 21.0 b |
| PVS2 60 min + LN | 4.90 ± 0.44 a | 4.03 ± 0.39 ab | 0.91 ± 0.58 a | 0.80 ± 0.56 a | 79.0 a | 73.0 a |
1 Statistical analysis in each column was performed by ANOVA. Data followed by different letters are significantly different at p ≤ 0.05 by Bonferroni’s test.