Table 4.
First subculture after V cryo-plate procedure: morphological parameters of S. rebaudiana shoots, from control and from cryopreserved apical shoot tips and axillary shoot tips. (Means ± SD).
| Treatment |
1 Shoot Length (cm) |
Shoots/Explants (n°) |
Explants with Shoot (%) |
|||
|---|---|---|---|---|---|---|
| Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
|
| Control | 4.52 ± 0.70 a | 4.16 ± 0.37 a | 1.00 ± 0.74 a | 0.97 ± 0.75 a | 78.0 a | 71.4 a |
| PVS2 30 min + LN | 4.00 ± 0.51 b | 3.95 ± 0.42 a | 0.90 ± 0.57 a | 0.81 ± 0.70 a | 77.0 a | 66.6 a |
| PVS2 60 min + LN | 4.37 ± 0.78 a | 4.20 ± 0.48 a | 0.93 ± 0.60 a | 0.73 ± 0.52 a | 78.6 a | 70.0 a |
1 Statistical analysis in each column was performed by ANOVA. Data followed by different letters are significantly different at p ≤ 0.05 by Bonferroni’s test.