Table 5.
Second subculture after V cryo-plate procedure: morphological parameters of S. rebaudiana shoots, from control and from cryopreserved apical shoot tips and axillary shoot tips. (Means ± SD).
| Treatment |
1 Shoot Length (cm) |
Shoots/Explants (n°) |
Explants with Shoot (%) |
|||
|---|---|---|---|---|---|---|
| Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
Apical Shoot Tips |
Axillary Shoot Tips |
|
| Control | 6.69 ± 0.98 a | 6.52 ± 0.88 a | 1.50 ± 0.68 a | 1.43 ± 0.81 a | 100.0 a | 93.3 a |
| PVS2 30 min + LN | 7.19 ± 1.00 a | 6.43 ± 1.10 a | 1.53 ± 0.81 a | 1.40 ± 0.81 a | 96.6 a | 83.0 a |
| PVS2 60 min + LN | 7.21 ± 1.40 a | 6.44 ± 1.50 a | 1.56 ± 0.96 a | 1.40 ± 0.96 a | 93.3 a | 83.0 a |
1 Statistical analysis in each column was performed by ANOVA, Data followed by same letters indicate no significant difference at p ≤ 0.05 by Bonferroni’s test.