Figure 7.

TILT-123 replicates selectively in cancer cells in vitro. A549 lung adenocarcinoma cells, human primary hepatocytes, MRC5 fibroblasts, and human vascular endothelial HUVEC cells were infected with TILT-123, Ad5/3 replicative control virus (similar selection device as TILT-123 but no cytokine payload), wild-type adenovirus type 5 (Ad5 wt), or replication-incompetent Ad5/3-Luc1 virus or left uninfected as a control (mock). (A) Virus genomes and (B) infectious virus particles were analyzed from the infected cells 72 h after infection. TILT-123 transgenes TNFa (C) and IL-2 (D) were analyzed from cell culture supernatants.