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. 2021 Jan 27;10(2):120. doi: 10.3390/antibiotics10020120

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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SDS-PAGE profile of recombinant lactoferrin expression. Probiotic hosts with porcine or bovine lactoferrin expression plasmids were induced (+) or non-induced (−) by lactoferrin expression using nisin for 4 h. Cells lysates were supernatant after sonication disruption and were analyzed via SDS-PAGE. LF: standard control panel, 0.32 μg purified LF; M: molecular weight marker; lane 2 to 3: Lactobacillus delbrueckii/PLF; lane 4 to 5: Lactobacillus gasseri/BLF; lane 6 to 7: Bifidobacterium angulatum/PLF. Arrow indicates target lactoferrin with a molecular weight of about 80 kDa.