Figure 8.

Effects of recombinant human or bovine lactoferrin on the growth of three recombinants: L. gasseri/BLF, L. delbrueckii/HLF, and L. delbrueckii/BLF. The recombinant probiotic strains were grown in deMan–Rogosa–Sharpe (MRS) broth to an optimal optical density at 600 nm (OD₆₀₀) of 0.2. Then, theses bacteria were cultured in MRS medium with and without nisin-induced expression of recombinant human or bovine lactoferrin. The growth responses of each bacterium were measured by determining the optical density at different time intervals at room temperature (approximately 23–25 °C). The results are expressed as mean ± standard deviation and variations in growth curves of probiotic bacteria with and without nisin induction. * Significant differences in the probiotic growth with and without nisin induction (P < 0.05).