Figure 9.

Effect of fucosterol (Fct) at 5 µM alone and in combination with doxorubicin (Dox) at 0.1, 1, 2, and 5 µM, on the viability of MDA-MB-231 cells in monolayer–72 h (a) and 3D–96 h (b) assessed by the resazurin assay. Cells treated with 0.1% DMSO and Dox 5 µM were included as negative and positive controls, respectively. The percentages of cell viability are relative to the control and presented as mean + standard deviation of five independent experiments (each in triplicate). Square brackets indicate t tests with Sequential Bonferroni corrections (** p < 0.01, *** p < 0.001 and **** p < 0.0001).